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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon BS(-)
Intact vector size: 43.100
Type of vector: phage
Vector end: EcoRI
Vector end: HindIII
Cloning sites: EcoRI HindIII
Polylinker sites: NotI BamHI EcoRI EcoRV HindIII SalI XhoI
Cloning capacity: 8.9 kb
Construction: Charon 15, pBluescript KS M13(-)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
other: left arm, ->, 1-21225
other: pBluescript KS -, ->, 21226-24184
MCS: NotI...KpnI, ->, 21226-21315
promoter for in vitro transcription: T3, <-, 21327-21346
promoter: lac, <-, 21416-21444
replicon: pMB1, 21771-21771
marker(s): ampR, <-, 22529-23389
replicon: f1, ->, 23520-23976
promoter for in vitro transcription: T7, ->, 24140-24159
MCS: SacI...NotI, ->, 24172-24184
other: right arm, ->, 24185-43100
Cross references:
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Vector
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Tissue: retina, male, 67 yr.0 DNA: cDNA Insert size range: 0.4 - 1.2 kb Genome Equivalent: 0.0
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Comments
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Titer determined on E. coli LE392. cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
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References
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Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057
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