2Q1 (ATCC® 77419)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2Q1
Species Homo sapiens, human
Depositors F Kao
Origin
lymphoblast, normal, female (GM03714)
Applications
Microdissection library derived from human chromosome 2q35-37. Can be used for chromosome painting and for isolating probes specific for the 2q35-37 region.
Vector
Tissue: lymphoblast, normal, female (GM03714)
Chromosome: 2q35-q37
DNA: genomic
Digest: Complete
Insert size range: 0.05 - 0.8 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 20000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Microdissection library derived from human chromosome 2q35-37. Can be used for chromosome painting and for isolating probes specific for the 2q35-37 region.
Deposited as PCR products. These were digested with MboI before cloning.
Approximately 25% of the inserts are non-human.
The library has about 40-50% repetitive sequence clones which are human specific.
References

Yu J, et al. Construction and characterization of a region-specific microdissection library from human chromosome 2q35-q37. Genomics 14: 769-774, 1992. PubMed: 1427905

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