pCS19 (ATCC® 77409)

Applications: shuttle vectorvector for constructing genomic librariesvector permitting positive selection for inserts  /  Depositors: C Sengstag

Permits and Restrictions

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Designations pCS19
Depositors C Sengstag
Biosafety Level 1
Vector Information
Size (kb): 9.3440008163452150
Vector: pCS19 (plasmid)
Promoters: Promoter lambda PR
Construction: YRp7-PHO5, URA3, CENXV, cI, pBR322
Marker(s):URA3,ampR
Construct size (kb): 9.344000816345215
Features: marker(s): ampR, URA3
promoter: lambda PR
replicon: pMB1, ARS1
repressor gene: cI
terminator: trp
centromere: CEN15
Applications
shuttle vector
vector for constructing genomic libraries
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): PstI--6.8, 1.35, 1.2; BamHI--8.15, 1.2; EcoRI--4.9, 4.45.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--5.2, 6.4; BglII--0.6; EcoRI--0.0, 4.46; HindIII--0.84, 2.0, 4.21, 4.33; PstI--0.63, 1.8, 8.59; SalI--5.48; SmaI--0.9, 4.16; XbaI--2.43; XhoI--3.75.
Insertion into the BclI site inactivates the cI repressor, allowing expression of tetracycline resistance in recombinants.
Propagation in a dam- host is necessary for cleavage at the BclI site.
Shuttle vector for construction of genomic libraries that permits direct selection of recombinants.
The order of the major features in the plasmid is: EcoRI - ARS1 - SmaI - URA3 - XbaI - CEN15 - XhoI - BclI/cI - lambda PR - tetR - trp terminator - BamHI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sengstag C. The sequence of Saccharomyces cerevisiae cloning vector pCS19 allowing direct selection for DNA inserts. Gene 124: 141-142, 1993. PubMed: 8440476

Sengstag C, Hinnen A. The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual amino acid composition. Nucleic Acids Res. 15: 233-246, 1987. PubMed: 3029672

Shipped freeze-dried
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