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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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hepatocellular carcinoma Hep G2 cell line
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt10
Intact vector size: 43.340
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.6 kb
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: lambda
Cross references:
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Vector
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Tissue: hepatocellular carcinoma Hep G2 cell line DNA: cDNA Insert size range: 2 - 9 kb Genome Equivalent: 0.0
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Shipping Information
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Distributed: frozen (volume: 0.2 mL)
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Comments
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Constructed after size selection of cDNAs. Internal EcoRI sites were methylated and inserts were cloned using EcoRI (8mer) linkers.
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References
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Gowda K, et al. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene 207: 197-207, 1998. PubMed: 9511762
Gitt MA, et al. Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267: 10601-10606, 1992. PubMed: 1375225
Morgan DO, et al. Insulin-like growth factor II receptor as a multifunctional binding protein. Nature 329: 301-307, 1987. PubMed: 2957598
Jeffrey C Edman, personal communication
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