placenta >4 kb (ATCC® 77399)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: JC Edman

Permits and Restrictions

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Designations placenta >4 kb
Species Homo sapiens, human
Depositors JC Edman
Origin
placenta, full-term (sex unknown)
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt10
Intact vector size: 43.340
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.6 kb
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: lambda
Cross references:
Vector
Tissue: placenta, full-term (sex unknown)
DNA: cDNA
Insert size range: > 4 kb
Genome Equivalent: 0.0
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
Amplified and titer determined on E. coli LE392.
Constructed after size selection of cDNAs. Internal EcoRI sites were methylated and inserts were cloned using EcoRI (8mer) linkers.
References

Fischman K, et al. A murine fer testis-specific transcript (ferT) encodes a truncated fer protein. Mol. Cell. Biol. 10: 146-153, 1990. PubMed: 2294399

Jeffrey C Edman, personal communication

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