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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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telencephalon, embryonic day 15
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: E61
Intact vector size: 3.800
Type of vector: phagemid
Vector end: EcoRV
Vector end: NotI
Cloning sites: SacI XhoI XbaI SfiI SacII NotI EcoRI EcoRV HindIII ClaI SalI
Polylinker sites: SacI XhoI XbaI SfiI SacII NotI - stuffer - EcoRI EcoRV
HindIII ClaI SalI
Construction: pBluescript SK-
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
Cross references:
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Vector
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Tissue: telencephalon, embryonic day 15 DNA: cDNA Digest: Complete Insert size range: 0.3 - 10 kb Genome Equivalent: 0.0 Coverage: Clones for "complete" coverage: 100000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 mL)
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Comments
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The vector is a derivative of pBluescript SK- modified in the polylinker to minimize complementarity between single-stranded forms of SK- and KS- plasmids. In this vector, transcription from the T7 promoter gives the sense strand. Directionally cloned library.
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Classification
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Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
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References
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Porteus MH, et al. Isolation and characterization of a library of cDNA clones that are preferentially expressed in the embryonic telencephalon. Brain Res. Mol. Brain Res. 12: 7-22, 1992. PubMed: 1372074
Rubenstein JL, et al. Subtractive hybridization system using single-stranded phagemids with directional inserts. Nucleic Acids Res. 18: 4833-4842, 1990. PubMed: 2168539
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