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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 6.1999998092651370 Vector: pUN100 (plasmid) Promoters: Promoter lac Construction: pUC18 Marker(s):LEU2,ampR Construct size (kb): 6.199999809265137 Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
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Applications
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YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--3.1 (doublet); SacI--6.2; HindIII--6.2. YC-type shuttle vector permitting visual detection of recombinants by lacZalpha complementation.
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References
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Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604
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Related Products
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component of:ATCC 87637
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