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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.6999998092651370 Vector: pUN70 (phagemid) Promoters: Promoter lac Construction: pUC18 Marker(s):URA3,ampR Construct size (kb): 5.699999809265137 Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
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Applications
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YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; KpnI--5.2; BamHI--5.2; PvuII--5.0, 0.3. YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA).
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References
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Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604
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Related Products
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component of:ATCC 87637
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