pUN30 [pNN332] (ATCC® 77265)

Applications: YC-type (centromeric) shuttle vectormutation detectionshuttle vectorvector permitting production of single-stranded DNAvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pUN30 [pNN332]
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 5.3000001907348630
Vector: pUN30 (phagemid)
Promoters: Promoter lac
Construction: pUC18
Marker(s):TRP1,ampR
Construct size (kb): 5.300000190734863
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: M13+
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
mutation detection
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; KpnI--5.4; HindIII--3.7, 1.7.
YC-type shuttle vector permitting visual detection of recombinants by lacZ alpha complementation and production of ssDNA (in the same sense as lacZ mRNA).
Growth Conditions
Temperature: 37.0°C
References

Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene 70: 303-312, 1988. PubMed: 3063604

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Product Sheet
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