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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.7630000114440920 Vector: pFL64- (phagemid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 4.763000011444092 Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, f1, 2 micron
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Applications
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YE-type (episomal) shuttle vector shuttle vector vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--3.7, 1.1; ClaI--4.2, 0.5. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR - f1 ori.
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References
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Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827
Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730
Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361
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