pFL64+ (ATCC® 77213)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL64+
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 4.7630000114440920
Vector: pFL64+ (phagemid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 4.763000011444092
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, f1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--3.7, 1.1; ClaI--4.2, 0.5.
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR - f1 ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730

Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361

Shipped freeze-dried
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