pFL44L (ATCC® 77208)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL44L
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 6.0630002021789550
Vector: pFL44L (plasmid)
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 6.063000202178955
Features: marker(s): ampR, URA3
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.1; BglII--5.0, 1.1; ClaI--3.9, 2.25.
Contrary to published data, this vector does not permit visual detection of recombinants using the assay for alpha complementation.
Shows increased stability because of the presence of STB. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 4.0.
YE-type high copy number shuttle vector.
The order of the major features in this plasmid is: NotI/MCS/HindIII - URA3 - STB -2 micron ori - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730

Francois Lacroute, personal communication

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