pFL44S (ATCC® 77205)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector permitting visual detection of recombinants  /  Depositors: F Lacroute

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Designations pFL44S
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 4.3189997673034670
Vector: pFL44S (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 4.318999767303467
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; BglII--3.3, 1.1; ClaI--3.9, 0.5.
The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 13.4.
YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730

Shipped freeze-dried
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