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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.3189997673034670 Vector: pFL44S (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 4.318999767303467 Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1, 2 micron
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Applications
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YE-type (episomal) shuttle vector shuttle vector vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--4.4; BglII--3.3, 1.1; ClaI--3.9, 0.5. The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 13.4. YE-type high copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - 2 micron ori - pMB1 ori - ampR.
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References
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Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827
Chevallier MR, et al. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene 11: 11-19, 1980. PubMed: 7002730
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