pFL36 [YCpGMC112] (ATCC® 77202)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector permitting visual detection of recombinants  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL36 [YCpGMC112]
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 6.3689999580383300
Vector: pFL36 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):LEU2,ampR
Construct size (kb): 6.36899995803833
Features: insert detection: lacZ'
marker(s): ampR, LEU2
promoter: lac
replicon: pMB1, ARS-CEN6
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; BglII--3.7, 2.9; ClaI--3.9, 1.9, 0.8.
The loss per generation (percentage having lost all the plasmids after 20 h of non-selective growth) is 8.3.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - LEU2 - ARS-CEN6 - pMB1 ori - ampR.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Shipped freeze-dried
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