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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 3.799 Vector: pFL34 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 3.799 Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1
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Applications
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YI-type (integrating) shuttle vector integrating vector shuttle vector vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--3.9; BglII--2.8, 1.1; ClaI--3.9. YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation. The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - pMB1 ori - ampR.
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References
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Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470
Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029
Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589
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