pFL34 plasmid in E. coli (ATCC® 77200)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vectorvector permitting visual detection of recombinants  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL34 plasmid in E. coli
Depositors F Lacroute
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.799
Vector: pFL34 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 3.799
Features: insert detection: lacZ'
marker(s): ampR, URA3
promoter: lac
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.9; BglII--2.8, 1.1; ClaI--3.9.
YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - URA3 - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map
Product sheet