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Size (kb): 4.7849998474121090
Vector: pRS200 (phagemid)
Promoters: Promoter T3
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Construct size (kb): 4.784999847412109
Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac, T3, T7
replicon: pMB1, f1, ARSH4
YC-type (centromeric) shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PvuI--2.8, 2.0; HindIII--3.7, 1.1; EcoRI--3.5, 1.3; XbaI--3.4, 1.4; PvuII--4.3; 0.4.
YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
An EcoRI site in the TRP1-containing fragment (external to the coding sequence) was destroyed.
Modified from pRS314 (ATCC 77143)
by replacing the SmaI site in the MCS with BglII linkers. Permits double digestion of the vector to lower background when inserting Sau3AI fragments.
The order of the major features in this plasmid is: TRP1 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4.
Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
Philip Hieter, personal communication
Freeze dried Escherichia coli HB101 containing the plasmid.