Saccharomyces cerevisiae genomic library in E. coli (ATCC® 77162)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: P Hieter

Permits and Restrictions

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Designations Saccharomyces cerevisiae genomic library in E. coli
GenBank Definition

S.cerevisiae tim17 gene.

Saccharomyces cerevisiae (CND1) gene, complete cds.

Saccharomyces cerevisiae Pip1p (PIP1) gene, complete cds.

Saccharomyces cerevisiae Pip2p (PIP2) gene, complete cds.

Saccharomyces cerevisiae Pip3p (PIP3) gene, complete cds.

Saccharomyces cerevisiae (Ssu1p) SSU1 gene, complete cds.

Saccharomyces cerevisiae glycine decarboxylase (GCV2) gene,

GenBank Number

X77796

L35923

U17262

U17263

U17264

U20254

U20641

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors P Hieter
Applications
Researchers ordering this library from the ATCC will also receive the vector used to construct it.
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p366
Intact vector size: 8.479
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII BamHI SphI
Polylinker sites:
Construction: YCp50
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
centromere: CEN4
marker(s): ampR, LEU2
replicon: pMB1, ARS1
Cross references:
Vector
DNA: genomic
Digest: Partial
Insert size range: 9 - 12 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 1000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
Researchers ordering this library from the ATCC will also receive the vector used to construct it.
p366 is available as ATCC 77163.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Goodson HV, et al. Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J. Cell Biol. 133: 1277-1291, 1996. PubMed: 8682864

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