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Vector Information
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Size (kb): 11.4540004730224600 Vector: pYAC4 (YAC) Marker(s):TRP1,URA3,ampR,HIS3,SUP4 Construct size (kb): 11.45400047302246 Features: marker(s): HIS3
marker(s): SUP4
marker(s): TRP1
marker(s): URA3
marker(s): ampR
other: telomere
replicon: ARS1
replicon: pMB1
centromere: CEN4
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Applications
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YL-type (YAC) shuttle vector shuttle vector vector for constructing genomic libraries
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Comments
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Yeast artificial chromosome (YAC) vector for cloning EcoRI fragments. A 0.7 kb BamHI-XhoI fragment contains two copies of the Tetrahymena thermophila macronuclear telomere. This yeast artificial chromosome contains ARS1, CEN4 and Tetrahymena telomeric elements.
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References
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Burke DT, et al. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812, 1987. PubMed: 3033825
Wells RA, et al. Telomere-related sequences at interstitial sites in the human genome. Genomics 8: 699-704, 1990. PubMed: 2276741
Burke DT, Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194: 251-270, 1991. PubMed: 2005791
Kuhn RM, Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene 141: 125-127, 1994. PubMed: 8163163
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Shipping Information
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Distributed: freeze-dried
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