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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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hamster (CHO)/human lymphocyte 24HL5-4 hybrid cell line
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Applications
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Derivation and characterization of the 24HL5-4 cell line is described in Somatic Cell Mol.
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Vector
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Tissue: hamster (CHO)/human lymphocyte 24HL5-4 hybrid cell line Chromosome: 2 DNA: genomic Digest: Partial Insert size range: 15 kb average Genome Equivalent: 5.170000076293945 Coverage: Clones for "complete" coverage: 22600.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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Purity (human clones): 65%. Contaminants (hamster): 33.5%. Verified to have an average insert size of 15 kb based on NotI digestion of DNA purified from the library. Chromosomes for library construction were isolated using flow sorting. Genome equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 251 Mb per chromosome 2. Derivation and characterization of the 24HL5-4 cell line is described in Somatic Cell Mol. Genet. 13: 539-551, 1987 and Genomics 8: 614-622, 1990.
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References
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Trask BJ, et al. Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization. Somatic Cell Mol. Genet. 17: 117-136, 1991. PubMed: 2011792
Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608
Jeffrey A Garnes, personal communication
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