LL01NL01 (ATCC® 57784)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: JA Garnes

Designations LL01NL01
Species Homo sapiens, human
Depositors JA Garnes
Origin
hamster/human HHW1113 hybrid cell line
Vector
Tissue: hamster/human HHW1113 hybrid cell line
Chromosome: 1
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 1.480000019073486
Coverage: Clones for "complete" coverage: 23800.0
Media Medium 1592: SM buffer
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Purity (human clones): 61%. Contaminants (hamster): 32%.
Verified to have an average insert size of 15 kb based on NotI digestion of DNA purified from the library.
Chromosomes for library construction were isolated using flow sorting.
Genome equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 257 Mb per chromosome 1.
The HHW1113 cell line was obtained from NIGMS Human Genetic Mutant Cell Repository.
References

Trask BJ, et al. Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization. Somatic Cell Mol. Genet. 17: 117-136, 1991. PubMed: 2011792

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Jeffrey A Garnes, personal communication

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