LL12NS01 (ATCC® 57756)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions

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Designations LL12NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte 81P5d hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte 81P5d hybrid
Chromosome: 12
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 20000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Fair isolation of #12 from neighboring X-peak and hamster peaks.
Hamster impurity is 44% estimated by flow karyotype; 34% hamster impurity by plaque hybridization.
Total human DNA hybridizes to 34% of plaques.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 34.
References

Marvin Van Dilla, personal communication

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