LA15NS02 (ATCC® 57729)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions

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Designations LA15NS02
Species Homo sapiens, human
Depositors LL Deaven
hamster (CHO)/human lymphocyte 81P5d hybrid
Tissue: hamster (CHO)/human lymphocyte 81P5d hybrid
Chromosome: 15
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 3.700000047683716
Coverage: Clones for "complete" coverage: 17000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
About 2% hamster impurity by in situ hybridization to sorted chromosomes; about 22% hamster impurity by plaque hybridization.
The library LA15SN02, ATCC 57729, 3.7 genomic equivalents, is distributed together with LA15NS03, ATCC 57740, 4.0 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 23.

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

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