LA11NS02 (ATCC® 57726)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions

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Designations LA11NS02
GenBank Definition

Human catalase (CAT) gene, exon 1, 5' end.

Human lung Clara cells 10 kda secretory protein (CC10) gene, satellite and Alu repeat sequences, complete cds.

GenBank Number

L13609

U01102

Species Homo sapiens, human
Depositors LL Deaven
Origin
hamster/human 80H10 hybrid cell line
Applications
This is preserved in a solution containing 10% glycerol and 2.0 M ClCl.
Vector
Tissue: hamster/human 80H10 hybrid cell line
Chromosome: 11
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 1.139999985694885
Coverage: Clones for "complete" coverage: 22000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
About 29% hamster impurity by in situ hybridization to chromosomes; about 27% hamster impurity by plaque hybridization.
About 20% hamster impurity.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 2.8.
This is preserved in a solution containing 10% glycerol and 2.0 M ClCl. The CsCl should be diluted to less than 40 mM before infection.
References

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

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