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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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skin fibroblast, male, HSF7 cell line
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Applications
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This is preserved in a solution containing 10% glycerol and 0.92 M CsCl.
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Vector
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Tissue: skin fibroblast, male, HSF7 cell line Chromosome: 9 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 1.399999976158142 Coverage: Clones for "complete" coverage: 23000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 7. This is preserved in a solution containing 10% glycerol and 0.92 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
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References
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Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
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