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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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hamster (CHO)/human lymphocyte 640-12 hybrid
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Applications
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This is preserved in a solution containing 10% glycerol and 0.27 M CsCl.
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Vector
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Tissue: hamster (CHO)/human lymphocyte 640-12 hybrid Chromosome: 5 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 6.599999904632568 Coverage: Clones for "complete" coverage: 30000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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This is preserved in a solution containing 10% glycerol and 0.27 M CsCl. The CsCl should be diluted to less than 40 mM before infection. About 4% hamster impurity by in situ hybridization of sorted chromosomes; about 25% hamster impurity by plaque hybridization. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 43.
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References
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Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
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