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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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GenBank Definition
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Human pyruvate dehydrogenase complex (PDHA2) gene, complete cds.
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Origin
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hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
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Applications
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This is preserved in a solution containing 10% glycerol and 2.53 M CsCl.
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Vector
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Tissue: hamster (CHO)/human lymphocyte UV20HL21-27 hybrid Chromosome: 4 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 2.299999952316284 Coverage: Clones for "complete" coverage: 31000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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This is preserved in a solution containing 10% glycerol and 2.53 M CsCl. The CsCl should be diluted to less than 40 mM before infection. The library LA04NS01, ATCC 57719, 1.6 genomic equivalents, is distributed together with LA04NSO2, ATCC 57718, 2.3 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 3.35.
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References
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Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
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