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Origin
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hamster (CHO)/human lymphocyte UV20HL4 hybrid
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Vector
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Tissue: hamster (CHO)/human lymphocyte UV20HL4 hybrid Chromosome: 11 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 4.900000095367432 Coverage: Clones for "complete" coverage: 22000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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The #11 peak was well-isolated from other human peaks, but neighboring hamster peaks (especially F) are close. This was a noisy preparation. About 48% of the library is human (and 52% hamster) by plaque hybridization. This library has been amplified once at the ATCC.
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References
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. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.
Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319
Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320
Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744
Marvin Van Dilla, personal communication
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