LL09NS01 (ATCC® 57703)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

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Designations LL09NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
hamster (CHO)/human lymphocyte UV41HL4 hybrid
Tissue: hamster (CHO)/human lymphocyte UV41HL4 hybrid
Chromosome: 9
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 13.0
Coverage: Clones for "complete" coverage: 23000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
The #9 peak was very well-isolated from other human peaks but some overlap with neighboring hamster peaks. By fluorescence in situ hybridization with sorted chromosomes, there is 17% hamster DNA.
About 62% of the library is human (and 38% hamster) by plaque hybridization.

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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