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Origin
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hamster (CHO)/human lymphocyte UV20HL21-17 hybrid
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Vector
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Tissue: hamster (CHO)/human lymphocyte UV20HL21-17 hybrid Chromosome: 8 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 24000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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The peak was well-isolated from hamster background; little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 15% hamster DNA. About 84% of the library is human (and 16% hamster) by plaque hybridization. Of 52 isolates giving definitive results, 5 contained hamster sequences. One hybridized to hamster (2 fragments) and human (same size as insert) DNA. Of 76 inserts recloned into pUC13: 8-no identifiable insert; 4-very small inserts; 38-human repeats; 3-identical to others; 2--lost; 5-hamster; 7-no hybridization; 9-human (2 multiple copy, 4/7 single copy detect RFLP, 1 on chromosome 4. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 93.
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References
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. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.
Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319
Wood S, et al. Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP). Am. J. Hum. Genet. 39: 744-750, 1986. PubMed: 2879441
Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320
Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744
Marvin Van Dilla, personal communication
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