LL08NS02 (ATCC® 57702)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions
Permits and Restrictions

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Designations LL08NS02
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
hamster (CHO)/human lymphocyte UV20HL21-17 hybrid
Vector
Tissue: hamster (CHO)/human lymphocyte UV20HL21-17 hybrid
Chromosome: 8
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 24000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
The peak was well-isolated from hamster background; little debris. According to fluorescence in situ hybridization using genomic hamster DNA with sorted chromosomes, there is 15% hamster DNA.
About 84% of the library is human (and 16% hamster) by plaque hybridization.
Of 52 isolates giving definitive results, 5 contained hamster sequences. One hybridized to hamster (2 fragments) and human (same size as insert) DNA.
Of 76 inserts recloned into pUC13: 8-no identifiable insert; 4-very small inserts; 38-human repeats; 3-identical to others; 2--lost; 5-hamster; 7-no hybridization; 9-human (2 multiple copy, 4/7 single copy detect RFLP, 1 on chromosome 4.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 93.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Wood S, et al. Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP). Am. J. Hum. Genet. 39: 744-750, 1986. PubMed: 2879441

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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