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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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GenBank Definition
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H.sapiens SOD-2 gene for manganese superoxide dismutase.
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Origin
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hamster (CHO)/human lymphocyte UV20HL15-33 hybrid
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Applications
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By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes.
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Vector
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Tissue: hamster (CHO)/human lymphocyte UV20HL15-33 hybrid Chromosome: 6 DNA: genomic Digest: Complete Insert size range: 4.0 kb average Genome Equivalent: 20.0 Coverage: Clones for "complete" coverage: 28000.0
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Shipping Information
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Distributed: frozen (volume: 0.2 ml)
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Comments
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The #6 peak overlaps hamster background; narrow window. By hybridization analysis, there is 15% hamster DNA in the sorted chromosomes. About 77% of the library is human (and 23% hamster) by plaque hybridization. The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 27.
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References
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. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.
Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319
Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320
Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712
Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744
Marvin Van Dilla, personal communication
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