pAL1 (ATCC® 57198)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: R Nusse

Permits and Restrictions

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Designations pAL1
GenBank Number

X03072

Species Homo sapiens, human
Depositors R Nusse
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.799999952316284
Tissue: placenta
Gene product: wingless-type MMTV integration site 1, human homolog [INT1]
Insert Size (kb) 2.800
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--4.4, 2.8; AccI--3.6, 1.9, 1.6; BamHI--7.2.
A new nomenclature system has been proposed for INT1 and related genes. It has been suggested that INT1 become WNT-1.
This probe contains exons 3 and 4.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, TaqI, MspI, BglII.
References

van Ooyen A, et al. The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences. EMBO J. 4: 2905-2909, 1985. PubMed: 2998762

van't Veer LJ, et al. Molecular cloning and chromosomal assignment of the human homolog of Int-1, a mouse gene implicated in mammary tumorigenesis. Mol. Cell. Biol. 4: 2532-2534, 1984. PubMed: 6513929

Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

Barbara Weiffenbach, personal communication

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