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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector
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Construct size (kb): 7.199999809265137
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Insert
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DNA: genomic Insert lengths(kb): 2.799999952316284 Tissue: placenta Gene product: wingless-type MMTV integration site 1, human homolog [INT1]
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Shipping Information
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Distributed: freeze-dried
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--4.4, 2.8; AccI--3.6, 1.9, 1.6; BamHI--7.2. A new nomenclature system has been proposed for INT1 and related genes. It has been suggested that INT1 become WNT-1. This probe contains exons 3 and 4. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, TaqI, MspI, BglII.
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References
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van Ooyen A, et al. The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences. EMBO J. 4: 2905-2909, 1985. PubMed: 2998762
van't Veer LJ, et al. Molecular cloning and chromosomal assignment of the human homolog of Int-1, a mouse gene implicated in mammary tumorigenesis. Mol. Cell. Biol. 4: 2532-2534, 1984. PubMed: 6513929
Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319
Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597
Barbara Weiffenbach, personal communication
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