pAL1 (ATCC® 57198)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: R Nusse

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Designations pAL1
GenBank Number


Species Homo sapiens, human
Depositors R Nusse
Construct size (kb): 7.199999809265137
DNA: genomic
Insert lengths(kb): 2.799999952316284
Tissue: placenta
Gene product: wingless-type MMTV integration site 1, human homolog [INT1]
Insert Size (kb) 2.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--4.4, 2.8; AccI--3.6, 1.9, 1.6; BamHI--7.2.
A new nomenclature system has been proposed for INT1 and related genes. It has been suggested that INT1 become WNT-1.
This probe contains exons 3 and 4.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, TaqI, MspI, BglII.

van Ooyen A, et al. The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences. EMBO J. 4: 2905-2909, 1985. PubMed: 2998762

van't Veer LJ, et al. Molecular cloning and chromosomal assignment of the human homolog of Int-1, a mouse gene implicated in mammary tumorigenesis. Mol. Cell. Biol. 4: 2532-2534, 1984. PubMed: 6513929

Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319

Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597

Barbara Weiffenbach, personal communication

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