Chlamydophila pneumoniae ATCC ® 53592D™

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format	frozen 1 µg/mL in 1X TE buffer 100 µL per vial, shipped frozen
Storage Conditions	Store at -70°C or colder
Applications	It is suitable for use in PCR and other molecular bacteriology and virology applications.
Comments	Chlamydophila pneumoniaeDNA, in total DNA isolated from Hep-2 cells (ATCC® CCL-23) infected with Chlamydophila pneumoniae, strain AR-39 (ATCC® 53592). This product was prepared using methods known to inactivate infecting agent. It is suitable for use in PCR and other molecular bacteriology and virology applications.
Biosafety Level	1

Quality Control Specifications	Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month). Concentration is verified as >1 µg/mL DNA by Picogreen^® assay. Purity is confirmed by OD₂₆₀/OD₂₈₀ ratio (>1.7) of bulk DNA before dilution for product filling. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µL of product on a 0.8% agarose gel, visualized by Ethidium bromide staining. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers. Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.

Quality Control Specifications

Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
Concentration is verified as >1 µg/mL DNA by Picogreen^® assay.
Purity is confirmed by OD₂₆₀/OD₂₈₀ ratio (>1.7) of bulk DNA before dilution for product filling.
Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µL of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.
Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

DNA from Chlamydophila pneumoniae strain AR-39 (ATCC® 53592D™)