Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47047)

Strain Designations: DG105 [CMCC 2022]  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations DG105 [CMCC 2022]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Genotype thi-1 endA1 hsdR17 lacIq delta(lacZ)M15 supE44 dam-13::Tn9 F'
Type Strain no
Comments
Tn10 was lost spontaneously.
Occasionally streak onto minimal medium and reisolate single colonies to assure M13 sensitivity.
Derived from DG98 (MM294) by P1 transduction and chloramphenicol selection; isolated by D. Gelfand.
A host strain for the preparation of dam- M13 DNA.
Medium Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
Genotype thi-1 endA1 hsdR17 lacIq delta(lacZ)M15 supE44 dam-13::Tn9 F'
Name of Depositor JC Hunter-Cevera
References

Lawyer FC, et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264: 6427-6437, 1989. PubMed: 2649500

Marinus MG, et al. Insertion mutations in the dam gene of Escherichia coli K-12. Mol. Gen. Genet. 192: 288-289, 1983. PubMed: 6316110

Jennie C Hunter-Cevera, personal communication

Notice: Necessary PermitsPermits

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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