Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47017)

Strain Designations: DB1257  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations DB1257
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Genotype F- delta(gpt-proA)62 thr-34::Tn10 trp-89::Tn5 supF58 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 hisG4 argE3 hsdR rac- sbcB15 recB21 recC22 rpsL31 rfbD1 kdgK51 thi-1 tsx-33 lambda-
Type Strain no
Comments
Propagates lambda clones containing inverted repeat sequences.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- delta(gpt-proA)62 thr-34::Tn10 trp-89::Tn5 supF58 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 hisG4 argE3 hsdR rac- sbcB15 recB21 recC22 rpsL31 rfbD1 kdgK51 thi-1 tsx-33 lambda-
Name of Depositor AR Wyman
References

Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567

Wyman AR, et al. Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts. Proc. Natl. Acad. Sci. USA 82: 2880-2884, 1985. PubMed: 3157994

Wyman AR, et al. Factors which equalize the representation of genome segments in recombinant libraries. Gene 49: 263-271, 1986. PubMed: 2952554

Ruther U, et al. pUR222, a vector for cloning and rapid chemical sequencing of DNA. Nucleic Acids Res. 9: 4087-4098, 1981. PubMed: 6272193

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation