Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43601-FZ)

Strain Designations: 7322  /  Type Strain: no  /  Biosafety Level: 2

Strain Designations 7322
Isolation
Feces, human
Belgium
Type Strain no
Biosafety Level 2
Antigenic Properties Serogroup I
Product Format frozen
Shipping Information Frozen
Preceptrol® no
Type Strain no
Antigenic Properties Serogroup I
Comments
Preparation of Clostridium difficile strain 7322 ATCC® 43601-FZ™, frozen for ease of use. Lot specific titers are available upon request.
Toxinotype tcdA-, tcdB-
Ribotype 031
Binary toxin gene cdtB not amplified by PCR
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture 80% N2 -10% CO2 -10% H2
Name of Depositor M Delmee
Isolation
Feces, human
Belgium
References

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

feces of child with diarrhea

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986