Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43594)

Strain Designations: W1194  /  Type Strain: no  /  Biosafety Level: 2

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Strain Designations W1194
Application
Enteric Research
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
Type Strain no
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serogroup A
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Antigenic Properties Serogroup A
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Toxinotype 0
Ribotype 005
Toxins
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Genotype Toxinotype: 0
Name of Depositor M Delmee
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation