Clostridium difficile (Hall and O'Toole) Prevot (ATCC® 43594)

Strain Designations: W1194  /  Type Strain: no  /  Biosafety Level: 2

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Strain Designations W1194
Application
Enteric Research
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
Type Strain no
Biosafety Level 2
Antigenic Properties Serogroup A
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Type Strain no
Antigenic Properties Serogroup A
Comments
Presence of tcdA and tcdB genes confirmed by PCR.
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Toxinotype 0
Ribotype 005
Toxins
Toxin B production confirmed by cytotoxicity assay using Vero cells (Cell line ATCC CCL-81).
Medium Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic gas mixture, 80% N2-10% CO2-10% H2
Genotype Toxinotype: 0
Name of Depositor M Delmee
Isolation
Feces of patient with pseudomembranous colitis, Belgium.
References

Delmee M, et al. Serogrouping of Clostridium difficile strains by slide agglutination. J. Clin. Microbiol. 21: 323-327, 1985. PubMed: 3980688

Delmee M, et al. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J. Clin. Microbiol. 24: 991-994, 1986. PubMed: 3782463

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation