Escherichia coli (Migula) Castellani and Chalmers (ATCC® 39403)

Strain Designations: JM103  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations JM103
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F' traD36 proA+ proB+ lacIq delta(lacZ)M15 delta(pro-lac) supE hsdR endA1 sbcB15 thi-1 rpsL lambda-
Type Strain no
Comments
Host strain E. coli JM103. Ten milliter of YT broth is inoculated with a single colony isolate. The overnight culture is diluted 1:10 into YT broth, and incubated for twelve hours and the supernatant is harvested.
Medium ATCC® Medium 2975: M9 Minimal Agar + 40ug/ml Streptomycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F' traD36 proA+ proB+ lacIq delta(lacZ)M15 delta(pro-lac) supE hsdR endA1 sbcB15 thi-1 rpsL lambda-
Name of Depositor JC Grosch
References

Hanahan D. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580, 1983. PubMed: 6345791

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation