lambdaS2T (ATCC® 37546)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LB Klickstein

Designations lambdaS2T
GenBank Definition

Human mRNA for complement receptor type 1 (CR1, C3b/C4b receptor, CD35).

GenBank Number

Y00816

Species Homo sapiens, human
Depositors LB Klickstein
Origin
tonsil
Applications
The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA size-selected on agarose gels was used in the construction.
Vector
Tissue: tonsil
DNA: cDNA
Insert size range: 2 - 7 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 400000.0
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
EcoRI linkers were added (with internal sites protected). The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA size-selected on agarose gels was used in the construction.
Actin clones comprise 0.3% of all recombinants. Proteins for which cDNAs have been isolated from this library include complement receptors CR1, CR2, and B chain of CR3, and leukocyte common antigen LCA.
References

Wong WW, et al. Identification of a partial cDNA clone for the human receptor for complement fragments C3b/C4b. Proc. Natl. Acad. Sci. USA 82: 7711-7715, 1985. PubMed: 2933745

Klickstein LB, et al. Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristic of C3/C4 binding proteins. J. Exp. Med. 165: 1095-1112, 1987. PubMed: 2951479

Kishimoto TK, et al. Cloning of the beta subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene family. Cell 48: 681-690, 1987. PubMed: 3028646

Benoit E De Crombrugghe, personal communication

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