lambdaT (ATCC® 37545)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LB Klickstein

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Designations lambdaT
Species Homo sapiens, human
Depositors LB Klickstein
Origin
tonsil
Applications
The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA was used in construction.
Vector
Tissue: tonsil
DNA: cDNA
Insert size range: 1.2 kb average
Coverage: Clones for "complete" coverage: 400000.0
Biosafety Level 1
Comments
EcoRI linkers were added (with internal sites protected). The cDNA was synthesized by oligo(dT) priming and 100 ng of DNA was used in construction.
Actin clones comprise 0.2% of all recombinants. Proteins for which cDNAs have been isolated from this library include CR1, CR2, LCA, B1, IgG, and tubulin.
This is the second amplification of this library.
References

Weis JJ, et al. Identification of a partial cDNA clone for the C3d/Epstein-Barr virus receptor of human B lymphocytes: homology with the receptor for fragments C3b and C4b of the third and fourth components of complement. Proc. Natl. Acad. Sci. USA 83: 5639-5643, 1986. PubMed: 3016712

Streuli M, et al. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J. Exp. Med. 166: 1548-1566, 1987. PubMed: 2824653

Tedder TF, et al. Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes. Proc. Natl. Acad. Sci. USA 85: 208-212, 1988. PubMed: 2448768

Benoit E De Crombrugghe, personal communication

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