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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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lymphocyte, Con A/PMA-stimulated, 15 hr
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Applications
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EcoRI linkers were used in the construction.
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Vector
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Tissue: lymphocyte, Con A/PMA-stimulated, 15 hr DNA: cDNA Insert size range: > 0.2 kb Genome Equivalent: 0.0 Coverage: Clones for "complete" coverage: 200000.0
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Comments
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Amplified on E. coli Y1088. Titer on Y1090. Wild-type (non-recombinants) lambdagt11 will amplify more than recombinants. The ratio after first amplification changed from 80% recombinant to 50% recombinant. EcoRI linkers were used in the construction.
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References
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Young RA, Davis RW. Yeast RNA polymerase II genes: isolation with antibody probes. Science 222: 778-782, 1983. PubMed: 6356359
Reeves R, et al. Molecular cloning of a functional bovine interleukin 2 cDNA. Proc. Natl. Acad. Sci. USA 83: 3228-3232, 1986. PubMed: 3486415
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