pUC118 (ATCC® 37462)

Applications: vector permitting production of single-stranded DNA  /  Depositors: P Dwivedi

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations pUC118
Depositors P Dwivedi
Biosafety Level 1
Vector Information
Size (kb): 3.2000000476837160
Vector: pUC118 (phagemid)
Promoters: Promoter lac
Construction: M13, pUC18
Marker(s):kanR
Construct size (kb): 3.200000047683716
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1, M13
Applications
vector permitting production of single-stranded DNA
Comments
A pUC derivative containing the IG (intergenic) region of M13 for production of ssDNA.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 153: 3-11, 1987. PubMed: 3323803

Shipped FD
Shipping Information

Shipped:  Freeze dried stock of Escherichia coli containing the plasmid.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map