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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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spinal cord, 1 da old infant
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Applications
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We suggest the use of Y1088 ( ATCC 37195) for amplification, Y1089 ( ATCC 37196) for immunological screening of lysogens, and Y1090 ( ATCC 37197) for immunological screening of plaques. |
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Vector
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Tissue: spinal cord, 1 da old infant DNA: cDNA Insert size range: > 0.8 - 1.0 kb Genome Equivalent: 0.0
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Comments
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We suggest the use of Y1088 ( ATCC 37195) for amplification, Y1089 ( ATCC 37196) for immunological screening of lysogens, and Y1090 ( ATCC 37197) for immunological screening of plaques. RNA was prepared by the guanidinium thiocyanate method, fractionated by oligo(dT) cellulose, linkered with EcoRI, and sized by agarose gel electrophoresis. The libraries are likely to contain some cDNAs of hnRNAs. This is the second amplification of this library.
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References
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Kamholz J, et al. Identification of three forms of human myelin basic protein by cDNA cloning. Proc. Natl. Acad. Sci. USA 83: 4962-4966, 1986. PubMed: 2425357
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