LMG3 (ATCC® 37433)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: RA Lazzarini

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Designations LMG3
GenBank Definition

Human a7 nicotinic acetylcholine receptor mRNA.

GenBank Number

L25827

Species Homo sapiens, human
Depositors RA Lazzarini
Origin
basal ganglia, 1-day-old infant, less than 6 hr autolysis
Applications
We suggest the use of Y1088 (ATCC 37195) for amplification, Y1089 (ATCC 37196) for immunological screening of lysogens, and Y1090 (ATCC 37197) for immunological screening of plaques.
This library has been used to isolate a cDNA for cholinesterase (E.C. 3.1.1.8).
Vector
Tissue: basal ganglia, 1-day-old infant, less than 6 hr autolysis
DNA: cDNA
Insert size range: > 0.8 - 1.0 kb
Genome Equivalent: 0.0
Biosafety Level 1
Comments
We suggest the use of Y1088 (ATCC 37195) for amplification, Y1089 (ATCC 37196) for immunological screening of lysogens, and Y1090 (ATCC 37197) for immunological screening of plaques.
RNA was prepared by the guanidinium thiocyanate method, fractionated by oligo(dT) cellulose, linkered with EcoRI, and sized by agarose gel electrophoresis. The libraries are likely to contain some cDNAs of hnRNAs.
This library has been used to isolate a cDNA for cholinesterase (E.C. 3.1.1.8).
This library has been amplified once at low density.
References

Kamholz J, et al. Identification of three forms of human myelin basic protein by cDNA cloning. Proc. Natl. Acad. Sci. USA 83: 4962-4966, 1986. PubMed: 2425357

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