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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 7.9499998092651370 Vector: YCp50 (plasmid) Construction: YIp5, YCp19 Marker(s):URA3,ampR,tetR Construct size (kb): 7.949999809265137 Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
replicon: ARS1
replicon: pMB1
centromere: CEN4
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Applications
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YC-type (centromeric) shuttle vector plasmid shuffling vector for constructing genomic libraries
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Comments
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YC-type shuttle vector. YCp50 was constructed by removing the EcoRI site in YCp19 between ARS1 and CEN4 and cloning the PvuII/HindIII fragment into the PvuII site of YIp5. The PvuII site was not regenerated.
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References
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Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818
Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795
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Shipping Information
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Shipped: Freeze dried E. coli HB101 containing the plasmid
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