YCp50 (ATCC® 37419)

Applications: YC-type (centromeric) shuttle vectorplasmid shufflingvector for constructing genomic libraries  /  Depositors: MD Rose

Permits and Restrictions

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Designations YCp50
Depositors MD Rose
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.9499998092651370
Vector: YCp50 (plasmid)
Construction: YIp5, YCp19
Marker(s):URA3,ampR,tetR
Construct size (kb): 7.949999809265137
Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
replicon: ARS1
replicon: pMB1
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
plasmid shuffling
vector for constructing genomic libraries
Comments
YC-type shuttle vector.
YCp50 was constructed by removing the EcoRI site in YCp19 between ARS1 and CEN4 and cloning the PvuII/HindIII fragment into the PvuII site of YIp5. The PvuII site was not regenerated.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

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Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried  E. coli HB101 containing the plasmid

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