YCp50 (ATCC® 37419)

Applications: YC-type (centromeric) shuttle vectorplasmid shufflingvector for constructing genomic libraries  /  Depositors: MD Rose

Designations YCp50
Depositors MD Rose
Biosafety Level 1
Vector Information
Size (kb): 7.9499998092651370
Vector: YCp50 (plasmid)
Construction: YIp5, YCp19
Marker(s):URA3,ampR,tetR
Construct size (kb): 7.949999809265137
Features: marker(s): URA3
marker(s): ampR
marker(s): tetR
replicon: ARS1
replicon: pMB1
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
plasmid shuffling
vector for constructing genomic libraries
Comments
YC-type shuttle vector.
YCp50 was constructed by removing the EcoRI site in YCp19 between ARS1 and CEN4 and cloning the PvuII/HindIII fragment into the PvuII site of YIp5. The PvuII site was not regenerated.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

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Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried  E. coli HB101 containing the plasmid

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