pEMBL8+ (ATCC® 37396™)

Applications: vector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: G Cesareni

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations pEMBL8+
Depositors G Cesareni
Biosafety Level 1
Vector Information
Size (kb): 3.9389998912811280
Vector: pEMBL8+ (phagemid)
Construction: pUC8, bacteriophage f1
Marker(s):ampR
Construct size (kb): 3.938999891281128
Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Applications
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.0; EcoRI--4.0; AccI--4.0; ClaI--4.0; AccI/ClaI--3.1, 1.0.
ssDNA-producing plasmid with polylinker in lacZ'.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771

Shipped FD
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map