|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Other IDs
|
Nucleotide (GenBank) :
VB0032
pUC18c cloning vector (beta-galactosidase mRNA on complementary strand).
Nucleotide (GenBank) :
VB0025
pUC18 cloning vector.
|
|
Vector Information
|
Size (kb): 2.686 Vector: pUC18 (plasmid) Promoters: Promoter lac Construction: pUC71K Marker(s):ampR Construct size (kb): 2.686 Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
MCS: HindIII...EcoRI
|
|
Applications
|
expression vector vector permitting construction of fusion proteins
|
|
Comments
|
Expression vector encoding the beta-galactosidase alpha peptide (lacZ'), permitting blue-white visual detection of re combinants.
|
|
References
|
Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259-268, 1982. PubMed: 6295879
Norrander J, et al. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26: 101-106, 1983. PubMed: 6323249
|
|
Shipping Information
|
Shipped: Freeze dried plasmid in E. coli
|