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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Other IDs
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Nucleotide (GenBank) :
VB0065
pSV2-Cat cloning vector.
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Vector Information
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Size (kb): 5.1 Vector: pSV2-cat (plasmid) Promoters: Promoter SV40 Construction: pSV2-betaG, Tn9 Marker(s):ampR,cmlS Construct size (kb): 5.1 Features: marker(s): ampR, cmlS
promoter: SV40
replicon: pMB1
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Applications
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expression vector shuttle vector
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Comments
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Restriction digests of the vector give the following sizes (kb): HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0. Expresses chloramphenicol acetyltransferase in eukaryotic cell lines under the control of the simian virus 40 early promoter.
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References
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Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240
Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651
Gorman CHigh efficiency gene transfer into mammalian cellsIn: Gorman CDNA cloning2Washington, DCIRL Presspp. 143-190, 1985
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