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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.729 Vector: pSV2-neo (plasmid) Promoters: Promoter SV40 early Construction: SV40, Tn5, pBR322 Marker(s):G418R,ampR Construct size (kb): 5.729 Features: marker(s): G418R
marker(s): ampR
promoter: SV40 early
replicon: SV40
replicon: pMB1
restriction site: HindIII
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Applications
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expression vector
shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet). Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
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References
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Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831
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Shipping Information
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Shipped: Frozen glycerol stock of E. coli containing the plasmid
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