YRp7 [pRB16] (ATCC® 37060)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YRp7 [pRB16]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 5.817
Vector: YRp7 (plasmid)
Promoters: none
Construction: pBR322, lambdagt-Sc4101
Marker(s):TRP1,ampR,tetR
Construct size (kb): 5.817
Features: marker(s): TRP1
marker(s): ampR
marker(s): tetR
promoter: none
replicon: ARS1
replicon: ROP
replicon: pMB1
terminator: none
enhancer: none
Applications
YR-type (replicating) shuttle vector
shuttle vector
Comments
A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YRp7 was constructed by inserting a 1.4 kb EcoRI fragment containing TRP1 and ARS1 from lambdagt-Sc4101 into pBR322. The ARS1 fragment is in a 0.85 kb EcoRI-HindIII subfragment.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map