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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.2439999580383300 Vector: pACYC184 plasmid in E. coli (plasmid) Construction: p15A, pSC101, Tn9 Marker(s):cmlR,tetR Construct size (kb): 4.24399995803833 Features: marker(s): cmlR, tetR
replicon: p15A
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Low copy number plasmid vector containing the replication system of miniplasmid p15A.
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References
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Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110
Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533
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Shipping Information
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Shipped: Frozen glycerol stock of Escherichia coli containing the plasmid.
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