pACYC184 (ATCC® 37033)

Depositors: SN Cohen

Permits and Restrictions

View Permits

Designations pACYC184
Depositors SN Cohen
Biosafety Level 1
Host Escherichia coli PM191
Vector Information Vector size (kb): 4.244
Markers: cmlR, tetR
DNA Seq. Acc.: X06403

Type of vector: plasmid
Cloning sites: EcoRI HindIII BamHI SalI
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features):
marker(s): cmlR, tetR
replicon: p15A

Suggested host(s):
Escherichia coli chi-1776 (ATCC® 31244™)
Escherichia coli chi-1776 (ATCC® 31537™)
Escherichia coli chi-1776 (ATCC® 39234™)
Escherichia coli chi-1776 (ATCC® 53705™)
Escherichia coli C600 (ATCC® 23724™)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
Media Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map