pACYC184 (ATCC® 37033)

Depositors: SN Cohen

Permits and Restrictions

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Designations pACYC184
Depositors SN Cohen
Biosafety Level 1
Vector Information
Size (kb): 4.2439999580383300
Vector: pACYC184 plasmid in E. coli (plasmid)
Construction: p15A, pSC101, Tn9
Marker(s):cmlR,tetR
Construct size (kb): 4.24399995803833
Features: marker(s): cmlR, tetR
replicon: p15A
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9.
Low copy number plasmid vector containing the replication system of miniplasmid p15A.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110

Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map